Absolute Gene-ius

Chickens cough, coronaviruses persist, and molecular methods can reveal more than meets the eye.In this episode of Absolute Gene-ius, University of Calgary researcher Fathima Ishara Isham joins Lisa Crawford and Jordan Ruggieri to explore infectious bronchitis virus (IBV), a coronavirus that affects poultry health and can disrupt egg production. Ishara explains how IBV can move beyond the respiratory tract, why vaccination strategies differ for broilers and layers, and the practical challenges of animal studies involving large sample sets and multiple time points. She also walks through her study comparing qPCR and digital PCR for IBV detection, including quantification range, limit of detection, precision, specificity, and testing of real-world tissue, swab, air, water, and dust samples. Her findings highlight how digital PCR can detect low-level viral signals in samples that tested negative by qPCR, raising new questions about persistence and infectivity. In Career Corner, Ishara shares her path from Sri Lanka to Canada, the value of mentorship, and why saying “I don’t know” can be a strength. Plus, she recalls a proud cell-culture milestone and one illuminating immunofluorescence mistake. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.

Choosing between qPCR and digital PCR isn't about finding a winner, it's about asking the right scientific question. In this Science Snapshot, Jordan Ruggieri and Lisa Crawford revisit insights from researchers including Corbin Schuster, Nikhil Ram Mohan, Brandi Kiel Reese, Pavla Brachova, Nehemiah Seth Alvarez, Raquel Muñoz, Rounak Feigelman, and Brian Bahder to explore how qPCR and dPCR work together across a wide range of applications. From environmental DNA and pathogen surveillance to COVID viral RNAemia, reproductive biology, CAR-T cell monitoring, and assay development, each guest demonstrates why qPCR often provides the speed, flexibility, and throughput needed for discovery, while digital PCR delivers the analytical sensitivity, precision, and absolute quantification required for challenging targets. Together, these examples illustrate that the strongest workflows often combine both technologies, allowing researchers to move seamlessly from broad screening to highly confident quantitative measurements. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.

PCR gets amplified, quantified, partitioned, and multiplexed in this practical tour through three decades of molecular biology innovation. Dr. John Pfeifer, senior real-time PCR field application scientist at Thermo Fisher Scientific, joins Jordan and Lisa to explain how conventional PCR, qPCR, and digital PCR generate and interpret results differently. John describes real-time PCR as a versatile “Swiss army knife,” while digital PCR offers particular advantages for detecting rare targets against a high background. He then explores multiplexing, including how TaqMan assays use distinct fluorescent dyes to detect multiple targets in one reaction. The conversation covers the benefits of multiplexing for throughput, sample conservation, precision, and quality control, along with key considerations such as target abundance, Taq polymerase saturation, primer limitation, dye selection, mixed standard curves, and wet-lab validation. In Career Corner, John traces his scientific curiosity from childhood books and theoretical physics to genetics, molecular virology, and PCR. He also recalls a demonstration gone wrong, a surprisingly restorative qPCR presentation involving Charles the goat, and the sales-rep conversation that changed his career. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.

In this Science Snapshot, we explore copy number variation (CNV) and why accurately measuring gene copies is critical for modern research. Jordan Ruggieri and Lisa Crawford demonstrate how digital PCR has transformed DNA quantification using conversations with past Absolute Gene-ius guests including Dave Bauer, Dustin Rubinstein, Raquel Muñoz, Lee Ann Baxter-Lowe, Parker Wilson, Valeria Rangel, and Brian Bahder. They begin with the fundamentals of absolute quantification before exploring real-world applications in CRISPR genome editing, CAR-T cell expansion, transplant monitoring using donor-derived cell-free DNA, chromosomal mosaicism, leukemia research, and environmental genomics. Along the way, listeners learn why digital PCR provides advantages over traditional PCR approaches for measuring rare targets, validating edits, detecting low-abundance DNA, and producing confident quantitative results. Whether you're new to CNV analysis or already using digital PCR in your research, this episode offers practical perspectives from scientists applying these technologies every day. It's a fast-paced overview of why accurate DNA measurement continues to shape discoveries across molecular biology. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.

Some science stories start with a textbook. Donna Paznar’s started with a tick. In this episode of Absolute Gene-ius, Donna Paznar, head of Molecular Infection Diagnostics at the Medical University of Vienna, discusses her work studying Streptococcus pneumoniae and its impact across the Austrian population. She explains why this respiratory bacterium can be especially dangerous for infants and older adults, how children may act as asymptomatic reservoirs, and why tracking more than 100 bacterial serotypes is both important and technically challenging. Donna also walks through the molecular methods behind her work, including qPCR for identifying positive samples, nested PCR for targeting more specific DNA regions, and sequencing to help confirm serotypes. Along the way, she highlights the complexity of surveillance research and the goal of developing more efficient multiplex qPCR approaches. The career corner takes a fun turn into ticks, workplace cliques, qPCR mistakes, and a proud sleuthing moment involving a rare tick-borne pathogen. Donna’s advice is simple and useful: try opportunities, expect failure, and stay open-minded. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.