In this episode of Chromatography Experts, Patrick Endres hosts Elena Kumm and Balu Guduri, as they discuss the challenges of moving from process development to GMP manufacturing and the growing demand for large-scale pre-packed chromatography columns. Topics include: The challenges of scaling from development to manufacturing When column packing becomes a business and regulatory risk The performance data behind the SkillPak™ 1k MAX launch Chromatographic consistency, robustness, and reproducibility at 1 L scale The value of pre-packed columns compared to in-house or third-party packing Early customer feedback and what success looks like for manufacturing-scale chromatography Whether you're involved in process development, scale-up, or downstream purification, this conversation provides valuable insights into reducing risk and improving confidence in manufacturing-ready chromatography solutions.
In this episode of Chromatography Experts, Patrick Endres of Tosoh Bioscience is joined by Steffi Stephen and Daniele Addis from Axolabs for an in-depth discussion on oligonucleotides. Together, they explore what oligonucleotides are, how they are produced, and the key steps involved in moving from synthesis to a purified therapeutic product. The conversation covers emerging applications such as antibody-oligonucleotide conjugates (AOCs), as well as the purification and analytical challenges associated with developing oligonucleotide-based therapies. Steffi and Daniele share insights into the critical role chromatography plays in addressing these challenges. Whether you're new to oligonucleotides or looking to expand your knowledge of this rapidly growing field, this episode offers valuable perspectives on the technologies, challenges, and partnerships driving the future of nucleic acid therapeutics.
In this episode of Chromatography Experts, we take a deep dive into the science and advantages of pre-packed chromatography columns. Our experts discuss how column packing impacts performance, reproducibility, and process efficiency, and why pre-packed solutions continue to gain traction in bioprocessing and pharmaceutical manufacturing. Topics covered include: The GMP advantages of pre-packed chromatography columns • What defines a "well-packed" column and why packing quality is critical • The science behind column packing and chromatographic performance • How pre-packed columns help improve consistency, reduce risk, and streamline operations • Key considerations when selecting a chromatography column for your process Subscribe to Chromatography Experts for more conversations on the science, technologies, and innovations shaping modern chromatography.
Getting started with MALS doesn’t have to be complex. In this episode of the Chromatography Experts podcast, we break down the fundamentals of Multi-Angle Light Scattering (MALS), from instrumentation and setup to real-world troubleshooting and data interpretation. You’ll learn: What MALS is and how it integrates with chromatography systems The detectors and setup required for accurate measurements How sample quality impacts your results (and common pitfalls to avoid) Key troubleshooting strategies when results don’t look right Best practices for maintaining performance and interpreting data with confidence Whether you're new to MALS or looking to refine your workflow, this episode is packed with practical insights to help you get consistent, high-quality results. Interested in learning more about MALS? Find more here: https://www.tosohbioscience.com/US-EN-separations/lens-mals-education?utm_source=social-organic&utm_
We are back with another episode of Chromatography Experts! This time, we focus on Multi-Angle Light Scattering (MALS) with Corey Meadows and Landry Bennett. In this episode, we break down the fundamentals of MALS, explore real-world applications, and explain why light scattering remains such a powerful analytical technique.