Microbial Chemistry

LC-MS; Chapters: 0:00 - Assignment results; 08:00 - Assignment results 2; 15:30 - Example; 17:15 - LC-MS; 20:00 - Electro spray; 23:30 - Assignment; 25:45 - APCI; 27:45 - APPI; 28:15 - MALDI; 29:45 - Micromass LCT; 31:15 - LC-MS Ttechniques; 33:34 - Example; 38:45 - Molecules being ionized;

Mass spectrometry; Chapters: 0:00 - Assignment explanation; 03:15 - Task; 04:00 - Example; 06:00 - Assignment 1; 08:30 - Assignment 2; 09:00 - Mass spectrometry; 14:00 - LC-MS; 19:30 - Mass spectrometers; 24:15 - Monoisotopic masses; 27:45 - Mass defect; 29:30 - Ethanol; 41:00 - Example; 43:45 - Ochratoxin A;

Detectors other than MS; Chapters: 0:00 - Introduction; 00:45 - Multiple detection; 04:15 - The DAD (PDA) detector; 17:15 - Chromatogram; 19:00 - Comparison of commercial HPLC detectors; 20:15 - Light scattering detector; 22:15 - Fluorescence detection; 28:30 - Example; 29:00 - Evaporative light scattering detector; 31:45 - Format of microtitre plates; 34:00 - Cytotox profile; 35:30 - Assignment;

More reactions involved in polyketide biosynthesis; Chapters: 0:00 - Introduction; 01:30 - Reductions of the polyketide chain; 04:45 - A typical PKS configuration; 11:30 - Reductions; 13:34 - Keto-enol tautomerism; 17:15 - Keto-enol tautomerism + reduction; 19:30 - C-alkylations by SAM; 23:30 - C-alkylations; 26:45 - Oxidations; 30:00 - Other ring formations; 39:45 - Recap;

Exercises explained; Chapters: 0:00 - Exercise 1; 03:00 - Exercise 2; 07:30 - Biosynthesis of deoxyerythronolide (part 1); 12:00 - Find the polyketide backbone of the following molecules...;

Biosynthesis of polyketide natural products; Chapters: 0:00 - Introduction; 00:45 - Aims and objectives; 04:45 - Biosynthesis of natural products; 08:00 - Biosynthesis of natural products; 10:00 - Making the polyketide chain; 15:45 - Coenzyme A; 29:30 - The missing link; 32:15 - Folding of the poly-beta-ketoester (C-C bond); 40:15 - Claisen or aldol;

Chromatographic separation; Chapters: 00:00 - Exercise; 02:00 - Impact of injection-solvent on RP separation of phosphorolipids; 03:30 - Column lifetime; 05:15 - Gradients; 07:00 - Equilibrium time; 08:30 - Exercise; 09:00 - Practical staff;

Chromatographic separation; Chapters: 00:00 - Part 3 More about HPLS system; 00:45 - The chromatogram; 01:15 - The HPLC system - binary; 03:00 - The gradient in HPLC; 05:30 - Loop injection; 08:15 - Normal gradient pressure curve; 10:00 - Modern UHPLC binary pump; 12:30 - Jet Weaver; 15:00 - Autosampler; 21:30 - Plate slope; 22: 00 - Particle diameter; 24:00 - Temperature-linear flow velocity; 24:45 - Scaling in chromatography; 30:00 - Examples; 33:00 - Pre-columns (guard columns); 34:45 - Poor injection; 37:00 - Example of injection in wrong solvent; 38:30 - Assignment;

Chromatographic separation; Chapters: 00:00 - Exercises;

Chromatographic separation; Chapters: 00:00 - Exercise; 09:00 - Part 2 Separation based on polarity; 16:00 - More polar PR phases; 20:45 - Hydropiloc interaction chromatography; 22:15 - Poll 2; 27:30 - Poll 3; 32:00 - Correlation between logD and retention; 33:45 - Exercise;

Chromatographic separation; Chapters: 0:00 - Introduction; 01:00 - Poll 1; 06:00 - Part 1 Chromatographic separation; 06:30 - Lecture introduction; 08:00 - Functional group chemistry; 12:45 - Orthogonal separation; 14:00 - Exploiting functionalities; 15:30 - Chromatography; 21:00 - SEM of Kinetex; 22:30 - Separation based on polarity; 24:45 - Functional groups affect polarity; 27:00 - pH can affect polarity; 32:15 - Types of chromatography; 36.45 - Exercise;