The Single-Cell World Podcast

Join us on this podcast episode as I explore the fascinating world of single-cell RNA sequencing (scRNA-seq) data normalization with Amartya Singh, a post-doctoral researcher at Reuters. Discover the limitations of the standard methods used in scRNA-seq data normalization and how Amartya's innovative solution is changing the game. Hear firsthand about his journey of inspiration and the ideas that led to the development of this groundbreaking approach. Don't miss out on this insightful discussion - tune in now! Useful Links: Amartya Singh LinkedIn and GitHub profile The starting point publication  TuBA paper Piccolo paper Support our podcast: pay us a Ko-fi

In this insightful podcast interview with Kieren Patel, the Chief Business Officer at Resolve Biosciences, we delve into the world of Molecular Cartography technology. Kieren provides details about this innovative platform, underscoring its remarkable capabilities in facilitating high molecular resolution pathology. Additionally, Kieren sheds light on forthcoming solutions set to be commercialized later this year, with a particular emphasis on their commitment to multi-omics and customized approaches. Tune in to gain valuable insights into the latest advancements in molecular cartography and discover the promising future that Resolve Biosciences' offerings hold.   Useful links: Resolve Biosciences website LinkedIn Twitter Youtube Datasets Kieren Patel Support our podcast: pay us a Ko-fi

In this podcast episode, I had the privilege of talking with Melanie Masuda, the Director of Market Development at Scale Biosciences. Melanie told me all about Scale Biosciences' cutting-edge technology and gave me a full overview of all of their different kits.   Listen now and understand how the single-cell (sc) sequencing DNA methylation, the scRNAseq, and the CRISPR enrichment kits work. Maybe this is the solution for your research project!   Useful Links: Melanie Masuda LinkedIn Scale Biosciences(website) Twitter LinkedIn KITS scDNA methylation scRNA CRISPR guide enrichment kit Support our podcast: pay us a Ko-fi  

In this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family. Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research.  In addition, we also talked about other common methods and the bigger picture of single-cell sequencing technology, such as the problems it faces and its future. Explore the fascinating world of SMART-seq methods with Simone Picelli - a must-listen episode. Tune in now and learn about the ideal way to study RNA isoforms.  ----more---- USEFUL LINKS: Simone Picelli Information LinkedIn Laboratory Publications SMART-Seq SMART-Seq 2 Original Paper SMART-Seq 2 Method Paper SMART-seq 3 SMART-Seq 3 Express FLASH-seq Protocols: FLASH-Seq FLASH-Seq UMI FLASH-Seq low amplification Bulk FLASH-Seq Scientist.com: science as a service Support our podcast: pay us a Ko-fi

The Single-Cell World Podcast is now available in video as well, but just on Apple Podcast and in our new YouTube channel  😉 Episode 14: Single-Cell Methods, an overview, needed an update since there is a fourth method that is being used to individualize cells when using single-cell sequencing technology. Join me and listen to it, as well as other updates concerning the methods that are at the core of the single-cell sequencing technology. Here I leave you a gift, related with this episode. Thank you for listening. Kind Regards, Cátia

Hi there! Today is Part 2 of the review of single-cell-related products launched in 2023. I hope I don't miss any. If I do, send me a message telling me about it. 😉 Here are some useful links: Exploring Single-Cell Sequencing online course Companies that I talk during this episode (part 2): Scale Biosciences Scipio Bioscience Singleron Biotechnologies Takara Bio 10X Genomics Free Resources Sponsorship Pay me a Ko-Fi Thanks for listening! Cheers, Cátia

Hi there! Today I review single-cell-related products launched in 2023. They are a lot! I hope I don't miss any. If I do, send me a message telling me about it. 😉 Here are some useful links: Join the Single-Cell World Newsletter Companies that I talk during this episode (part 1): BD Biosciences  Ep. 18: Samples Multiplexing BioScryb Genomics Fluent BioSciences Episode 31: Podcast Fluent BioSciences Honeycomb Biotechnologies Episode 19: Podcast Honeycomb Biotechnologies Mission Bio Episode 16: Podcast Mission Bio Parse Biosciences  Episode 35: Podcast Parse Biosciences Free Resources Sponsorship Pay me a Ko-Fi Thanks for listening! Cheers, Cátia

Hello there! The Spatial and Single-Cell Analysis Playbook was released last week. Front Line Genomics produced it. I was honored to be invited to take part in it. In this episode, you can hear the questions that were asked (AI voice here) and my responses during this fantastic interview. I hope you like it! Useful Links Free Resources The Single-Cell World website Sponsorship Pay me a Ko-Fi Thanks for listening. Cheers, Cátia

Hi there! Would you like to know what quality control steps I think are essential before a spatial transcriptomics experiment? In my experience, these control steps can save you a lot of headaches. Listen now, and tell me if you agree. Useful Links The Single-Cell World website Sponsorship Pay me a Ko-Fi Thanks for listening. Cheers, Cátia

Discover the marvels of MERSCOPE®, a revolutionary platform that's reshaping the world of single-cell spatial transcriptomics. Think of it as Google Earth for genes; it allows us to directly map and quantify the spatial distribution of hundreds to tens of thousands of RNA species in individual cells. Dr. Jiang He, Vizgen’s Scientific Co-founder and Senior Director of Scientific Affairs explains everything about the MERSCOPE® technology!    Listen now to learn more about single-cell spatial technology. And if you enjoy our podcast, don't forget to share with friends and subscribe to our podcast to listen to more groundbreaking discussions! 🚀🧬   Useful links: Vizgen website MERSCOPE Platform Vizgen Twitter, LinkedIn, Youtube Dr. Jiang He Twitter and LinkedIn   Support This Podcast Sponsorship Pay me a Ko-Fi Thanks for listening. Cheers, Cátia

Hi! Would you like to get a general idea about the sample preparation standard protocols for spatial transcriptomics experiments? Listen now 😉. Useful links: Youtube Videos Frozen Tissues Preparation FFPE Tissues Preparation How to Cut Your Frozen Tissue Sample To Get Beautiful Sections? OCT Blocks Prep Support this podcast Ko-fi Sponsors Thank you so much for listening. Cheers, Cátia

Welcome to this episode, in which I have a fascinating conversation with Charles Roco, the founder and CTO of Parse Biosciences. In our conversation, Charlie talks about Evercode single-cell technology. We look into the amazing flexibility of this technology, which allows us to go beyond the limits of gene expression analysis. Join us as we examine this intriguing high-throughput technology that 96-well plates allow for. Parse Biosciences useful links: Website Resources: application notes, whitepapers, data sets,etcetera.. LinkedIn Twitter Instagram Charles Roco LinkedIn and Twitter Support this podcast by buying me a coffee! Single-Cell World Website Thanks for listening! Have a lovely day, Cátia

Hi tere, Do you know that besides solid tissues, we can use other types of samples for Spatial Trancriptomics? Listen now to this episode podcast to know about all the sample types that can be used. Useful links: Support this podcast by buying me a coffee! Single-Cell World Website Thanks for listening! Have a lovely day, Cátia

In this episode, Dr. Elizabeth Tseng, associate director product manager at PacBio, explains everything about the MAS-Seq kit, a game-changer for producing long-read libraries. With the backing of single-cell industry giant 10X Genomics, the MAS-Seq kit promises to redefine the future of single-cell RNA seq assays. Whether you're a scientist or just curious about the future of single-cell research, this episode is your guide to the cutting-edge advancements in the field. Tune in to learn, explore, and be amazed! Some interesting links: Dr. Elizabeth Tseng, Social Media: X and LinkdeIn PacBio Resources: Website PacBio Single-Cell RNA sequencing MAS-seq Protocol MAS-seq Original Paper Webinars Usefull Links Support this podcast Join Our Newsletter New Website Thanks for listening! Cheers, Cátia

Hi! This is our first live episode on Instagram: Spatial Trancriptomics Image-Based Methods🎙️ In this live, I talk about the different methods that are in the base of some of the Spatial Transcriptomics technology. Listen now! Usefull Links Support this podcast Join Our Newsletter New Website Thanks for listening! Cheers, Cátia

Hi there, In this episode, I talk with Kristina Fontanez, PhD, co-founder and Senior Vice president of Product Development at Fluent Biosciences. We talk about the groundbreaking PIPseq single cell technology. Step into the fascinating world of single-cell RNA sequencing as Kristina demystifies the revolutionary PIPseq method that is making waves in single-cell research. What makes PIPseq stand out? Tune in now! Fluent Biosciences useful links: Website Resources: application notes, whitepapers, data sets, etc.. LinkedIn Twitter Kristina Fontanez LinkedIn Support this podcast by buying me a coffee! Single-Cell World Website Thanks for listening! Have a lovely day, Cátia

Hi! This is our first live episode on LinkedIn: Spatial Trancriptomics Methods🎙️ In this live, I talk about the two main categories of Spatial Transcriptomics methods. Listen now! Usefull Links Support this podcast New Website Thanks for listening! Cheers, Cátia

Today I decided to do something different. In this episode I share with you some frequent questions that I get on my talks and scientific consultations. Besides tell you that today is the last day to join our online course: Exploring the Cutting-Edge World of Single-Cell Technology And that next week I will be live on LinkedIn. In the meanwhile, luck for your single-cell experiments! Support this podcast Thanks for listening! Cheers, Cátia

First Live Episode on X: Introduction to Spatial Omics 🎙️ Get ready for an authentic experience—this is our debut live episode, streaming directly on X (Twitter)! Just a heads-up 👉🏻: we're keeping it real with unedited sound. In this unique live edition, we delve into the topic of Spatial Omics, focusing specifically on the fundamental reasons driving the development of this transformative technology. Don't miss it! Usefull Links Join now our online course: Exploring the Cutting-Edge World of Single-Cell Technology Support this podcast New Website Thanks for listening! Cheers, Cátia

In this episode, Dr. Ming “Tommy” Tang shares his story of changing from hands-on lab work to computer-based research. Tommy talks about the steps, challenges, and skills he learned along the way. Plus, find out how he got so many followers on X! This episode is not about methods or technology; it's about inspiring people in the single-cell research world. Give it a listen and get inspired by Tommy’s story. It can be a change in your own science journey! Some interesting links: Dr. Ming Tommy Tang Social Media X LinkdeIn Chatomics Youtube Channel Website: Diving into Genetics and Genomics Book: From Cell Line to Command Line Other Resources Support this podcast Thanks for listening! Cátia