This Week in Microbiology

TWiM explains how ticagrelor alters the membrane of S. aureus and enhances the activity of vancomycin and daptomycin without eliciting cross-resistance, and the development of a novel continuous disinfectant technology that decreases healthcare-associated infections in ICUs by 70%. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson. Become a patron of TWiM. Links for this episode Ticagrelor and S. aureus (mBio) Novel disinfectant technology (Am J Inf Control) UVC-LED to inactivate foodborne pathogens (Appl Envir Micro) UV disinfection systems (ACS Photonics) High-touch surfaces in specialized patient care area (CDC) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv  

TWiM focuses on recent foodborne outbreaks of bacterial infections, and how nanopore sequencing technology can be used to identify pathogenic microbes and antimicrobial resistance genes in food products. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Foodborne outbreaks (CDC) Race to nourish a warming world (Gates Foundation) Nanopore sequencing of foods (Food Microbiol) How is Oxford Nanopore used? (YouTube) Introduction to Nanopore sequencing (YouTube) Methods for detecting foodborne pathogens (Appl Micro Biotech) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM travels to the U.S. Army Medical Research Institute of Infectious Diseases to learn how research conducted at USAMRIID leads to vaccines, drugs, diagnostics, and training programs that protect both warfighters and civilians. Hosts: Michael Schmidt, Petra Levin and Michele Swanson. Guests: Norman Kreiselmeir, Christopher K Coat, Keersten Ricks, and Eric Nguyen Links for this episode: U.S. Army Medical Research Institute of Infectious Diseases Threading the NEIDL (TWiV 200) Unintentional importation of B. pseudomallei into US (Emerg Inf Dis) Become a patron of TWiM. Links for this episode Engineering the cow for less methane emissions (WaPo) Precision microbiome editing (Audacious Project) Giant viruses carry antibiotic resistance genes (Nat Commun) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explains a project to engineer the cow microbiome to reduce emissions of methane, and the finding of antibiotic resistance genes in the genomes of giant viruses. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Links for this episode Engineering the cow for less methane emissions (WaPo) Precision microbiome editing (Audacious Project) Giant viruses carry antibiotic resistance genes (Nat Commun) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explains how bacterial community structure can be used to predict athletic performance in racehorses, and the idea that a tiny fraction of all species forms most of Nature. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Mark O. Martin. Become a patron of TWiM. Links for this episode Microbiome picks a winner (Sci Rep) Picking a Winner by Reading the Form Hen’s Teeth and Horse’s Toes by Stephen Jay Gould How much does it cost to breed a horse? Date of birth and purchase price as foals or yearlings and race performance Rarity as a sticky state (PNAS) How many species on Earth? (PLoS Biol) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explains unique modifications in the energy conservation pathways linked to methanogenesis in an Archaeon, and mechanisms of white nose fungal invasion of cells from the Little Brown Bat. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Methyl-reducing methanogenesis (Nature) Pathogenic strategies of Pseudogymnoascus destructans (Science) Adaptive fungal invasion of bat cells (Science) Little brown bat (Critter Catalog) Nature Notes: Little Brown Bat (Harpswell) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM describes experiments to explore gut microbiota signatures of vulnerability to food addiction in mice and humans, and how a phage tail-like protein suppresses competitors in populations of bacteria of plants. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Gut microbiota and food addiction (Probiotics) Blautia may have probiotic properties (Gut Microbes) Blautia wexlerae ameliorates obesity and type 2 diabetes (Nat Commun) Phage tail–like bacteriocin suppresses competitors (Science) What is a bacteriocin? (Front Micro) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explores evolution and host adaptation of Pseudomonas infections of plants, and the impact of COVID-19 on ESBL-producing E. coli on urinary tract and blood infections. Hosts: Vincent Racaniello and Michael Schmidt. Become a patron of TWiM. Links for this episode Evolution and host adaptation of Pseudomonas (Science) Type III secretion system, infection by injection (Nat Comm) Demographic inference with skyline plots (Peer J) Skyline plots (Taming the Beast) Panaroo, a bacterial genome analysis pipeline (Wellcome Sanger Inst) Impact of COVID-19 on ESBL-producing E. coli infections (Antimicro Resist Inf Control) Take the TWiM Listener survey! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explores the deep-dwelling microbes that sculpt our planet, and the use of microbes in bioelectronics to manage inflammation. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Deep-dwelling microbes that sculpt our planet (NY Times) Living bioelectronics resolve inflammation (Science) Active biointegrated living electronics for managing inflammation (Science) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

From ASM Microbe in Atlanta, Georgia, Arturo joins TWiM to reveal the threats that fungi pose to human health, including the notorious Candida auris and many more and how committed experts are researching ways to save us and our food supplies. Hosts: Michael Schmidt, Mark O. Martin Guest: Arturo Casadevall Watch this episode: https://youtu.be/nKJe5xNUocU Become a patron of TWiM. Links for this episode Disaster mycology (Biomedica) Emergence of C. auris (mBio) What if fungi win? (JHU Press) Thinking about Science: Good Science, Bad Science, and How to Make It Better (Amazon) Recorded at ASM Microbe 2024 in Atlanta, Georgia. Join us at the next ASM Microbe by visiting us at asm.org/microbe Matters Microbial Take the TWiM Listener survey! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explains a new mechanism for preventing lysogeny through temperate phage-antibiotic synergy, and Salmonella expansion in the murine gut dependency on aspartate derived from reactive oxygen species-mediated microbiota lysis. Hosts: Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Temperate phage-antibiotic synergy (mBio) Salmonella expansion dependent on aspartate (Cell Host Micr) Arrowsmith by Sinclair Lewis (Wiki) A Genetic Switch by Mark Ptashne Lysis timing and bacteriophage fitness (Genetics) HK97 capsid assembly (Ad Exp Med Biol) Mode of action of fluoroquinolones (Drugs) Salmonella a foodborne pathogen (CDC) Freeman Hrabowski Scholars Program (HHMI) Sam Kaplan - 30 years of Microbiology (McGovern Medical School) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explores how climate change may be increasing our risks to infectious disease and then how the Odyssey literally comes alive in our microbial world but fear not, unlike the Trojans, the bacteria are fighting back and have developed resistance to this novel class of newly developed antimicrobials.    Become a patron of TWiM. Links for this episode: Environmental changes fueling diseases (NY Times) Global change drivers and risk of infectious diseases (Nature) First reported cefiderocol-resistant E. coli in Canada (Clin Micro) E. coli cells explode (YouTube) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM explores the plasticity of the adult human small intestinal stoma microbiota, and survival and rapid resuscitation that permit limited productivity in desert microbial communities. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Links for this episode Plasticity of small intestinal stoma microbiome (Cell Host Micr) Desert microbial communities (Nat Comm) How soil microbes survive in the desert (Science Daily) Negev Desert (WikiCommons) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

Today on TWiM, a charcuterie invasion, and how that acid in your stomach may protect from the invading hordes of microbes. Hosts: Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode 2024 Salmonella outbreak linked to charcuterie meats  Multitier regulation of the E. coli extreme acid stress response by CsrA Commentary: Peeling the onion: additional layers of regulation in the acid stress response Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reviews a case of E. faecium bacteremia treated with combination bacteriophage and antibiotic therapy, and how dopamine receptor D2 confers colonization resistance via microbial metabolites. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Guest: Mark O. Martin Become a patron of TWiM. Links for this episode Matters Microbial Distinct Fusobacterium dominates colorectal cancer (Nature) Bacterial subspecies that might drive colon cancer (Nature) A bacterial strain linked to colon cancer (Nature) Spatial perspective on bacteria in tumors (Nature) Colorectal cancer in the young (Yale Med) Surface colonization by Flavobacterium johnsoniae promotes its survival (mBio) THOR, a model microbiome (mBio) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reviews a case of E. faecium bacteremia treated with combination bacteriophage and antibiotic therapy, and how dopamine receptor D2 confers colonization resistance via microbial metabolites. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Links for this episode Vincent’s interviews at SXSW Bacteriophage and antibiotic therapy for E. faecium bacteremia (mBio) Dopamine receptor D2 confers colonization resistance (Nature) CDC’s Reports of Selected E. coli Outbreak Investigations Brett Finlay’s narrated EPEC animation Colonization resistance by gut microbial metabolome (ACS Chem Biol) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM discusses the identification of natural products from reconstructed ancient bacterial genomes, and how plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection. Hosts: Vincent Racaniello, Michael Schmidt, and Petra Levin. Become a patron of TWiM. Links for this episode Natural products from ancient bacterial genomes (Science) Plant mRNAs move into fungal pathogens (Cell Host Microb) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reviews the ongoing cholera outbreak in Africa, and research showing that gut complement induced by the microbiota blocks pathogens and spares commensal bacteria. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Cholera in Southern Africa (Africa CDC) Deadly cholera outbreak in Africa (NY Times) Pediatric cholera in sub-Saharan Africa (Curr Op Ped) Gut complement spares commensals (Cell) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reveals a new population in the blue cheese-making fungus Penicillium roqueforti and identification of a quorum-sensing autoinducer and siderophore in uropathogenic Escherichia coli. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Google Podcasts, Android, RSS, or by email. Become a patron of TWiM. Links for this episode New blue cheese-making fungus (Evol Appl) Threat to Camenbert cheese (Guardian) French Cheese Under Threat (CNRS News) Fungadapt project (YouTube) Microbes Make the Cheese (ASM) Yersiniabactin in uropathogenic Escherichia coli (mBio) Public goods and cheating in microbes (Curr Biol) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reveals a database of genome sequences of thousands of Mycobaterium tuberculosis, allowing association with resistance phenotypes to 13 antibiotics, and microbe-derived uremic solutes that enhance thrombosis potential in the host. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson. Become a patron of TWiM. Links for this episode M. tuberculosis genomes and antimicrobial resistance (PLoS Biol) The CRyPTIC consortium BashTheBug Zooniverse Microbial solutes enhance thrombosis (mBio) Can our microbiome break our heart? (mBio) Pathogenesis of cardiovascular disease (EJIFCC) How Kidneys Work Video (Mayo Clinic) What is a metaorganism? (Zoology) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv