️Episode 217: Multiscale triads of meiotic crossover patterning

In this episode of PaperCast Base by Base, we explore FFT and inverse-FFT analysis of Zip3/Zip2, Hop1 and Zip1 on yeast pachytene chromosomes reveals two interdigitated tiers of evenly spaced protein triads that correspond to canonical and minority crossovers and are differentially regulated by Pch2/TRIP13 Study

Highlights: Quantitative fluorescence profiles and Fourier analysis identify two dominant spatial periodicities centered near 0.5 µm and 1.0 µm for Zip3/Zip2, Hop1 and Zip1 along pachytene chromosomes. Inverse FFT reconstructs narrow and broad peaks that colocalize into triads of the three proteins with intra-triad separations of ~70 nm.

Shorter-periodicity triads map to canonical Zip3-defined crossovers and show classical interference, while longer-periodicity triads are interdigitated with shorter triads and also exhibit interference. The ratio, spacing relationships, and modulation by Pch2/TRIP13 support the interpretation that longer-periodicity triads correspond to minority crossovers and that Pch2 selectively alters abundance and relative loading in the longer-tier triads

Conclusion: Crossover interference establishes two interdigitated, multiscale patterns of tightly clustered Zip2/Zip3–Hop1–Zip1 triads along meiotic chromosomes, linking canonical and minority crossovers into a single interference-governed process

Music: Enjoy the music based on this article at the end of the episode.

Reference: White MA, Weiner B, Chu L, Lim G, Prentiss M & Kleckner N. Crossover interference mediates multiscale patterning along meiotic chromosomes. Nature Communications. 2025;16:10 453. https://doi.org/10.1038/s41467-025-65423-6

License: This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/

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