Generating libraries of diversified oligonucleotides, especially those with sequences containing randomized codon regions, is a critical step in the development of antibody, peptide, and nanobody libraries. These libraries are foundational tools in therapeutic discovery, enabling the identification of molecules with high specificity and affinity for a wide range of targets. The methods or technologies used for generating these libraries have evolved to allow high levels of diversity, specificity, and efficiency.

The currently available methods and techniques for generating such libraries are:

Solid-Phase DNA Synthesis

Trimer Phosphoramidite-Based Synthesis & Considerations Regarding Trimer Phosphoramidite Technology

PCR-based Random Mutagenesis

DNA Shuffling

Microchip-based DNA Synthesis

Oligonucleotide Ligation

CRISPR-Cas9 Mediated Library Generation

Cell-Free Transcription-Translation Systems

Phage Display with Randomized Peptide Libraries

All these will be discussed in detail in this podcast.

After which Library Cloning, Characterisation and Functionalization will be considered.

Stay curious, stay innovative. I'm Luke McLaughlin.

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