Susanne Heck (NIHR BRC): Introduction to Mass Cytometry (with English subtitles)
Update: 2019-06-04
Description
https://www.ibiology.org/techniques/mass-cytometry
How does mass cytometry differ from other types of flow cytometry? When would you choose to use it? How does a mass cytometer work? Dr. Susanne Heck gives an overview of mass cytometry and answers all of these questions.
Dr. Susanne Heck begins her talk by explaining why we might choose to use mass cytometry rather than other types of flow cytometry. Traditional flow cytometry is typically limited to the detection of about a dozen parameters in one sample due to overlap between the emission spectra of fluorochromes used to label antibodies. Mass cytometry, on the other hand, allows for the detection of up to 50 parameters in one sample because antibodies are labelled with metal isotopes and separated based on their mass. Heck goes on to explain which metal isotopes are typically used for mass cytometry and why, and she describes how a mass cytometer functions. She finishes by running through an example of using mass cytometry to perform functional phenotyping on human bone marrow cells.
Speaker Biography:
Dr. Susanne Heck received her PhD in molecular biology from the University of Bremen, Germany, in 1997. After a postdoc in molecular and cellular biology at Albert Einstein College in New York, Heck joined Cellular Genomics Inc., USA, to work on preclinical models for small molecule kinase inhibitors. In 2004, she moved to the Lindsey F. Kimball Research Centre to develop and run the Flow Cytometry Core of the New York Blood Centre. Heck was appointed as head of the NIHR BRC Flow Cytometry Core for Guys and St Thomas Hospital and King’s College London in 2009 and has established a successful human immune monitoring core of international reputation.
How does mass cytometry differ from other types of flow cytometry? When would you choose to use it? How does a mass cytometer work? Dr. Susanne Heck gives an overview of mass cytometry and answers all of these questions.
Dr. Susanne Heck begins her talk by explaining why we might choose to use mass cytometry rather than other types of flow cytometry. Traditional flow cytometry is typically limited to the detection of about a dozen parameters in one sample due to overlap between the emission spectra of fluorochromes used to label antibodies. Mass cytometry, on the other hand, allows for the detection of up to 50 parameters in one sample because antibodies are labelled with metal isotopes and separated based on their mass. Heck goes on to explain which metal isotopes are typically used for mass cytometry and why, and she describes how a mass cytometer functions. She finishes by running through an example of using mass cytometry to perform functional phenotyping on human bone marrow cells.
Speaker Biography:
Dr. Susanne Heck received her PhD in molecular biology from the University of Bremen, Germany, in 1997. After a postdoc in molecular and cellular biology at Albert Einstein College in New York, Heck joined Cellular Genomics Inc., USA, to work on preclinical models for small molecule kinase inhibitors. In 2004, she moved to the Lindsey F. Kimball Research Centre to develop and run the Flow Cytometry Core of the New York Blood Centre. Heck was appointed as head of the NIHR BRC Flow Cytometry Core for Guys and St Thomas Hospital and King’s College London in 2009 and has established a successful human immune monitoring core of international reputation.
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