BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

Update: 2007-09-26

Description

Outline:Assessing nanomedical system (NMS) targeting at the single cell levelFluorescent labeling of NMSsFirst estimates of NMS binding by fluorescence microscopyInternal of external binding by confocal microscopySingle-cell image/confocal analysisFlow cytometric quantitation of NMS binding to specific cell typesImage confocal analysis of NMS binding to single cellsAbility to scan/locate cells of interestPhotobleaching challengesOptical sectioning for 3D location of NMSs on/within cellsA quick overview of flow cytometryBasic principlesCapabilities and limitationsUse for assessing specificity and sensitivityRare-event analysis of NMS targeting to desired cellsBasic concepts of rare-event analysisStrategies for rare cell detectionMore advanced flow cytometry for ultra-rare cell detectionExamples of rare cell detectionRare cell sampling statistics

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In Channel

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BME 695N Lecture 21: FDA and EPA Regulatory Issues

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BME 695N Lecture 20: GMP and issues of quality control manufacture of nanodelivery systems

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BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use

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BME 695N Lecture 17: Assessing nanotoxicity at the single cell level

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BME 695N Lecture 16: Assessing drug efficacy at the single cell level

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BME 695N Lecture 15: Nanodelivery of therapeutic genes & molecular biosensor feedback control systems

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BME 695N Lecture 14: Challenges of proper drug dosing with nanodelivery systems

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BME 695N Lecture 13: Assessing Zeta Potentials

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BME 695N Lecture 12: Introduction to X-ray Photoelectron Spectroscopy and the Examples of Biologically-Relevant Applications

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BME 695N Lecture 11: Construction of biomolecule conjugated nanoparticles

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BME 695N Lecture 9: Atomic Force Microscopy (AFM) for Nanomedical Systems (cells and nanoparticles)

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BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

2007-09-26--:--

BME 695N Lecture 10: Nanomaterials for core design

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BME 695N Lecture 8: Technologies for measuring nanomedical systems on/within cells

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BME 695N Lecture 7: Normal & facilitated cell entry mechanisms

2007-09-16--:--

BME 695N Lecture 5: Cell Targeting

2007-09-12--:--

BME 695N Lecture 4: Designing "Theragnostic" Systems

2007-09-04--:--

BME 695N Lecture 3: Overview of Basic Nanomedical Systems Design

2007-08-29--:--

BME 695N Lecture 2: Basic Concepts of Nanomedical Systems

2007-08-28--:--

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BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

#box-pro-ellipsis-176070126614029{-webkit-line-clamp:2;}BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

James Leary

BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo