DiscoverExplain PodcastEpisode 1: Basics - from DNA to Sanger sequencing
Episode 1: Basics - from DNA to Sanger sequencing

Episode 1: Basics - from DNA to Sanger sequencing

Update: 2023-06-26
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Description

Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotide




Chapters:


00:00-Intro


02:00-DNA


08:27-Enzymes


13:05-RNAs, tRNAs


16:18-mRNAs, µRNAs, lncRNAs, circRNAs


21:35-Wobble


23:30-PCR


33:20-Sanger sequencing I


36:30-Gel electrophoresis


40:00-Sanger sequencing II


53:10-Teaser next Episode, Outro






Topics:




DNA, RNA, Protein, PCR, Gel Electrophoresis, Sanger Sequencing






- The Royal Institution on the DNA Discovery: https://www.youtube.com/watch?v=_w2453iom9s


- The discovery of DNA on science history: https://sciencehistory.org/education/scientific-biographies/james-watson-francis-crick-maurice-wilkins-and-rosalind-franklin/


- The New York Times on Franklins’ part: https://www.nytimes.com/2023/04/25/science/rosalind-franklin-dna.html


- Wikipedias article on Proteins with history: https://en.wikipedia.org/wiki/Protein


- Jrank on RNA: https://science.jrank.org/pages/5871/Ribonucleic-Acid-RNA-discovery-RNA.html


- Microbe Notes showing the central dogma of molecular biology: https://microbenotes.com/central-dogma-replication-transcription-translation/


- ThoughtCo on RNA and DNA: https://www.thoughtco.com/dna-versus-rna-608191


- khanacademy explainers on PCR and Gel electrophoresis: https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/polymerase-chain-reaction-pcr


- Amoeba Sisters on PCR: https://www.youtube.com/watch?v=a5jmdh9AnS4


- Promega explaining Sanger sequencing: https://www.youtube.com/watch?v=dVRB4CaLizc


- Microbe online’s writeup on Sanger sequencing: https://microbeonline.com/dna-sequencing-sanger-sequencing-method/


- LibreTextsBiology on Proteins and Enzymes: https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Book%3A_General_Biology_(Boundless)/03%3A_Biological_Macromolecules/3.07%3A_Proteins_-_Types_and_Functions_of_Proteins


- excedr on Taq Polymerase and PCR: https://www.excedr.com/resources/taq-polymerase-what-is-it-and-what-does-it-do/








Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.


Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).




The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).




Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).




P.S.: One DNA nucleotide is ~0.34 nanometers in „length“ - DR

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Episode 1: Basics - from DNA to Sanger sequencing

Episode 1: Basics - from DNA to Sanger sequencing

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