If ligation is more your thing

- Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms

- Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/

- Quick run-through video by ABI: https://www.youtube.com/watch?v=nlvyF8bFDwM

- Ars technica about the technology:
https://arstechnica.com/science/2009/12/dna-sequencing-gets-solid-with-built-in-error-detection/

- atdbio explaining SOLiD sequencing:
https://atdbio.com/nucleic-acids-book/Next-generation-sequencing#Sequencing-by-ligation-SOLiD

- Biogene Blog on SOLiD sequencers:
https://www.creative-biogene.com/blog/index.php/2017/02/22/the-next-generation-sequencing-platform-of-abi-company/

Chapters:

00:00 Intro

02:45 Ligation

06:50 Getting hairy balls

20:00 Bead 1 with DNA polonies

23:30 6 Base Jokers

24:00 2-Base encoding colours

29:15 Offsets and holes

35:00 Randomness and barcodes

37:25 Chemistry summary

40:30 Homopolymers are not the problem

46:30 SOLiDs fate

Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.

Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).

The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).

Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).