H+ ions and where we can find them

Chapters:

00:00 Intro

04:26 pH

08:04 DNA Polymerase reaction with H+

12:30 Nucleotides

16:30 Homopolymer problem, but different

19:30 PCR is mean to some bases

24:30 AmpliSeq

33:40 Today’s status

38:58 cfDNA

40:00 PHRED vs Qscore

44:59 Chip scaling and prices

51:00 Outro

- bitesizedbio on the technology:
https://bitesizebio.com/27399/all-in-the-chip-ion-torrent-sequencers/

- Quick explainer from Basic Biochem: https://www.youtube.com/watch?v=vMUnN2Y48Us

- Slideshow giving an overview:
https://www.slideshare.net/USDBioinformatics/ion-torrent-sequencing

- biobinge explaining Iontorrent sequencing:
https://biobinge.pubpub.org/pub/its/release/1

- rna-seqblog showing a comparison to Illumina:
https://www.rna-seqblog.com/a-comparison-of-illumina-and-ion-torrent-sequencing-platforms-and-the-winner-is/

- Explainer by Shomu’s Biology: https://www.youtube.com/watch?v=0Ch5189aaCU

Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.

Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).

The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).

Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).