296-PRISM: Single-Round High-Multiplex Spatial RNA Imaging
Update: 2025-12-05
Description
The paper introduces PRISM (profiling of RNA in situ through single-round imaging), a new high-multiplex fluorescent in situ hybridization (FISH) method designed to make spatial RNA imaging more accessible and efficient by overcoming the limitations of conventional microscopy. PRISM expands coding capacity by using color-intensity barcoding within a single round of staining, allowing for up to 64-plex detection without complex, expensive fluidics-dependent instrumentation. The article demonstrates PRISM’s robustness by applying it to create detailed spatial and quasi-3D atlases of diverse tissues, including mouse embryos, mouse brain sections, and human hepatocellular carcinoma (HCC). These applications reveal intricate biological details, such as cellular heterogeneity, developmental progression, and the complex microscale organization of the HCC tumor microenvironment.
References:
- Chang T, Zhao S, Deng K, et al. High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes[J]. Nature Biotechnology, 2025: 1-13.
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